抗癌药物载体用棕榈酰嵌段聚(甲基丙烯酰氧乙基磷酰胆碱)聚合物胶束的合成与表征《Biomacromolecules》
Synthesis and Characterization of Palmitoyl-block-poly(methacryloyloxyethyl Phosphorylcholine) ...
Synthesis and Characterization of Palmitoyl-block-poly(methacryloyloxyethyl Phosphorylcholine) Polymer Micelles for Anticancer Drug Delivery
Miao Zhang, Zhongxing Zhang, Xia Song, Jingling Zhu, Jing An Sng, Jun Li*, and Yuting Wen*
Department of Biomedical Engineering, National University of Singapore, 15 Kent Ridge Crescent, Singapore 119276, Singapore
National University of Singapore (Chongqing) Research Institute, 2 Huizhu Road, Yubei District, Chongqing 401120, China
Biomacromolecules 2022, XXXX, XXX, XXX-XXX
Publication Date: September 14, 2022
https://doi.org/10.1021/acs.biomac.2c00838
Abstract
We report the synthesis and characterization of an amphiphilic polymer comprising a hydrophobic palmitoyl group and a zwitterionic poly(2-methacryloyloxyethyl phosphorylcholine) (pMPC) block, which is capable of forming micelles as a drug carrier system for delivering hydrophobic anticancer drugs such as doxorubicin (DOX). We hypothesize that the sharp polarity contrast between the palmitoyl (Pal) domain and the pMPC block would strengthen the micelles and improve the drug loading capacity, while the pMPC shells improve the micelle stability and cellular uptake efficiency. In this study, the Pal-pMPC polymer was characterized and compared with a Pal-poly(ethylene glycol) (Pal-PEG) polymer in terms of their micelle formation, cytotoxicity, and drug loading of DOX. The DOX-loaded Pal-pMPC micelles were further evaluated for the cellular uptake and anticancer activities in cell culture systems including the non-multidrug-resistance HeLa cell line and the multidrug-resistance AT3B-1 cell line. The results showed that the Pal-pMPC polymer had a minimal toxicity. The Pal-pMPC micelles exhibited higher drug loading capacity and enhanced cellular internalization efficiency compared to micelles formed by the Pal-PEG polymer. It was also found that DOX-loaded Pal-pMPC micelles exhibited a more efficient anticancer effect than Pal-PEG micelles in multidrug-resistance cancer cells in an environment with fetal bovine serum.
SUBJECTS: